The Subline of Metastatic Prostate Cancer Cell line, DU145 expresses Tks4 and reveals distinctive Phenotype

Cancer metastasis is the process of dissemination cancer cells from their original site and localization in distant sites. During this journey, metastatic have to undergo several phenotypical/ genotypical changes for acquiring cell migration, invasion, attachment properties etc. These steps can be partially/fully mimicked under vivo vitro conditions. Here, we injected prostate line, DU145, into chorioallantoic membrane )CAM( chicken embryo generate sublines which were isolated 7-days post-injection, subcultured Our results showed that one these sublines, DC-1-, exhibited a distinctive phenotype compared with parental line when grown 3D culture model. Further, expression Tks4 at mRNA protein level DC-1- was observed higher than regular DU145 cells. Keywords: Prostate cancer, Metastasis, Round, Stellate, Colony,